Different restriction enzyme-generated sticky DNA ends can be joinedin vitro
نویسندگان
چکیده
منابع مشابه
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We rlpscribe a simDle method for joining the 5'-protrudinq, single-stranded DNA ends generated by restriction enzymes. The method allows ends with different seouences to be joined and prevents identical ends from being joined. This is accomplished by partially filling the single strands in a controlled reverse transcriptase reaction. Partial filling can create new single-stranded ends that can ...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1984
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/12.4.1863